Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time

GE Lidgerwood; A Senabouth; CJA Smith-Anttila; V Gnanasambandapillai; DC Kaczorowski; D Amann-Zalcenstein; EL Fletcher; SH Naik; AW Hewitt; JE Powell; A Pébay

Journal article

Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time

GE Lidgerwood, A Senabouth, CJA Smith-Anttila, V Gnanasambandapillai, DC Kaczorowski, D Amann-Zalcenstein, EL Fletcher, SH Naik, AW Hewitt, JE Powell, A Pébay

Genomics Proteomics and Bioinformatics | Published : 2021

DOI: 10.1016/j.gpb.2020.08.002

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Abstract

Human pluripotent stem cell (hPSC)-derived progenies are immature versions of cells, presenting a potential limitation to the accurate modelling of diseases associated with maturity or age. Hence, it is important to characterise how closely cells used in culture resemble their native counterparts. In order to select appropriate time points of retinal pigment epithelium (RPE) cultures that reflect native counterparts, we characterised the transcriptomic profiles of the hPSC-derived RPE cells from 1- and 12-month cultures. We differentiated the human embryonic stem cell line H9 into RPE cells, performed single-cell RNA-sequencing of a total of 16,576 cells to assess the molecular changes of th..

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University of Melbourne Researchers

Grace Lidgerwood Author

Daniela Amann-Zalcenstein Author

Erica Fletcher Author

Shalin Naik Author

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Grants

Awarded by Macular Disease Foundation Australia

Funding Acknowledgements

This work was supported by a National Health and Medical Research Council (NHMRC-Australia) Practitioner Fellow-ship (awarded to AWH) , Career Development Fellowship (awarded to JEP) , and Senior Research Fellowship (Grant No. 1154389, awarded to AP) , an Australian Research Council Future Fellowship (Grant No. FT140100047, awarded to AP) , NHMRC project grants (Grant Nos. 1138253 awarded to ELF and AP, as well as 1062820 and 1124812 awarded to SHN) , a NHMRC synergy grant (Grant No. 1181010 awarded to ELF and AP) , grants from the Macular Disease Foundation Aus-tralia (awarded to AP, JEP, and AWH) , the Jack Brockhoff Foundation (awarded to GEL) , the DHB Foundation (awarded to GEL and AP) , the Ophthalmic Research Institute of Australia (awarded to AP and AWH) , Stem Cells Australia - the Australian Research Council Special Research Initiative in Stem Cell Science (awarded to SHN, AWH, JEP, and AP) , the TMG Family Fund (awarded to AP and GEL) , a donation from Ms Jacqueline Pascual, the University of Melbourne, and the Operational Infrastructure Support from the Victorian Government, Australia.